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RAB25 antibody (RAB25, Member RAS Oncogene Family)

Details for Product anti-RAB25 Antibody No. ABIN969377, Supplier: Log in to see
Antigen
  • AV012978
  • CATX-8
  • catx-8
  • MGC79587
  • MGC84182
  • RAB11C
  • rab25
  • RAB25
  • si:dkeyp-84f11.7
  • zgc:101648
Reactivity
Human, Mouse (Murine)
57
38
19
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
41
22
4
Clonality (Clone)
Monoclonal ()
Conjugate
This RAB25 antibody is un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
49
35
23
12
8
8
7
4
3
1
Supplier
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Immunogen Purified recombinant fragment of Rab25 expressed in E. coli.
Clone 3F12
Isotype IgG1
Purification Ascites
Alternative Name Rab25 (RAB25 Antibody Abstract)
Background Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
Molecular Weight 24 kDa
Gene ID 57111
HGNC 57111
Research Area Cancer
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-RAB25 antibody (RAB25, Member RAS Oncogene Family) (ABIN969377) Figure 1: Flow cytometric analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and...
Immunocytochemistry (ICC) image for anti-RAB25 antibody (RAB25, Member RAS Oncogene Family) (ABIN969377) Figure 2: Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-RAB25 antibody (RAB25, Member RAS Oncogene Family) (ABIN969377) Figure 3: Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) ...
Western Blotting (WB) image for anti-RAB25 antibody (RAB25, Member RAS Oncogene Family) (ABIN969377) Figure 4: Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and...
Product cited in: Wang, Kumar, Navarre, Casanova, Goldenring: "Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 29138-46, 2000 (PubMed).

Background publications Goldenring, Shen, Vaughan, Modlin: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).