CD63 antibody

Antigen: CD63

Clonality: Monoclonal (NK1-C3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96942

Additional Information

Immunogen: Smooth plasma membrane fraction of MeWo cells.

Format: Ig-fraction

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: NK1-C3

Specificity: This antibody is specific to 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 to 1:200 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied purified antibody containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human melanoma stained with CD63 antibody

Publications

Publications: Hagen, Vennegoor, Schlingemann et al.: "Correlation of histopathological characteristics with staining patterns in human melanoma assessed by (monoclonal) antibodies reactive on paraffin sections." in: Histopathology, Vol. 10, Issue 7, pp. 689-700, 1986 (PubMed).

Vennegoor, Ruemke: "Circulating melanoma-associated antigen detected by monoclonal antibody NKI/C-3." in: Cancer immunology, immunotherapy : CII, Vol. 23, Issue 2, pp. 93-100, 1986 (PubMed).

Palmer, Hall, Lew: "A comparison of some methods for identifying amelanotic and oligomelanotic melanoma metastases in paraffin sections." in: Pathology, Vol. 17, Issue 2, pp. 335-9, 1985 (PubMed).