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CHEK1 antibody (Checkpoint Kinase 1)

Details for Product anti-CHEK1 Antibody No. ABIN969476, Supplier: Log in to see
Antigen
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Alternatives
anti-Human CHEK1 antibody for Immunoprecipitation
Reactivity
Human, Mouse (Murine)
451
149
140
5
4
4
3
2
2
2
1
1
Host
Mouse
394
50
22
6
Clonality (Clone)
Monoclonal ()
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
ELISA, Western Blotting (WB)
374
179
138
64
60
34
25
22
20
8
6
5
1
1
Supplier
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Immunogen Purified recombinant fragment of CHK1 expressed in E. coli.
Clone 2G1D5
Isotype IgG1
Purification Purified
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Molecular Weight 56 kDa
Gene ID 1111
HGNC 1111
Research Area Kinases/Phosphatases, Cell Cycle
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN969476) Figure 1: Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN969476) Figure 2: Western blot analysis using CHK1 mouse mAb against HEK293T cells transfecte...
Background publications Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Product cited in: OConnell, Raleigh, Verkade, Nurse: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).