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NEDD8 antibody

NEDD8 Reactivity: Human WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 1A7 unconjugated
Catalog No. ABIN969546
  • Target See all NEDD8 Antibodies
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    Reactivity
    • 66
    • 29
    • 25
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 64
    • 4
    • 3
    Mouse
    Clonality
    • 60
    • 11
    Monoclonal
    Conjugate
    • 30
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEDD8 antibody is un-conjugated
    Application
    • 38
    • 26
    • 26
    • 13
    • 13
    • 13
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Purification
    purified
    Immunogen
    Purified recombinant fragment of human NEDD8 expressed in E. coli.
    Clone
    1A7
    Isotype
    IgG1
  • Application Notes
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified antibody in PBS with 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    4°C, -20°C for long term storage
  • Ohki, Funatsu, Konishi, Chiba: "The mechanism of poly-NEDD8 chain formation in vitro." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 443-7, (2009) (PubMed).

  • Target
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    Alternative Name
    NEDD8 (NEDD8 Products)
    Synonyms
    NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody
    Background

    Description: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

    Aliases: NEDD-8

    Molecular Weight
    9 kDa
    Gene ID
    4738
    HGNC
    4738
    Pathways
    Ubiquitin Proteasome Pathway
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