Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody

Antigen: Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA)

Synonyms: PI3K, MGC142161, MGC142163, p110-alpha, C-P3K, PIK3CA, pi3k, MGC116320

Clonality: Monoclonal (4F3)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN969555

Additional Information

Alternative name: PIK3CA

Gene ID: 5290

Immunogen: Purified recombinant fragment of human PIK3CA expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 4F3

Description: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Synonyms: PI3K, p110-alpha

Molecular Weight: 124kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Purified

Buffer: PBS with 0.05% sodium azide and 0.5% protein stabilizer.

Preservative: 0.05% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Restrictions: For Research Use only

Publications

Product: Edwards, Witherspoon, Wang et al.: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, 2009 (PubMed).

Pang, Flinn, Patsialou et al.: "Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase." in: Cancer research, Vol. 69, Issue 23, pp. 8868-76, 2009 (PubMed).