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PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide)

Details for Product anti-PIK3CA Antibody No. ABIN969555, Supplier: Login to see
Antigen
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
Alternatives
anti-Human PIK3CA antibody for ELISA
Reactivity
Human
155
50
43
7
4
2
1
1
1
Host
Mouse
114
23
22
Clonality (Clone)
Monoclonal ()
Conjugate
This PIK3CA antibody is un-conjugated
7
7
7
6
5
5
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
91
75
67
33
22
10
9
6
5
3
2
2
1
1
Supplier
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Immunogen Purified recombinant fragment of human PIK3CA expressed in E. coli.
Clone 4F3
Isotype IgG1
Purification Purified
Alternative Name PIK3CA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Molecular Weight 124 kDa
Gene ID 5290
HGNC 5290
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 2: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and...
Immunocytochemistry (ICC) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green) . ...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 4: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) ...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 5: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 8...
Product cited in: Pang, Flinn, Patsialou et al.: "Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase." in: Cancer research, Vol. 69, Issue 23, pp. 8868-76, 2009 (PubMed).

Michelucci, Di Cristofano, Lami et al.: "PIK3CA in breast carcinoma: a mutational analysis of sporadic and hereditary cases." in: Diagnostic molecular pathology : the American journal of surgical pathology, part B, Vol. 18, Issue 4, pp. 200-5, 2009 (PubMed).

Edwards, Witherspoon, Wang et al.: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, 2009 (PubMed).