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Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody

Details for Product No. ABIN969555
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Antigen
Synonyms CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha, 6330412C24Rik, caPI3K, p110, p110alpha, C-P3K, PI3-kinase, PI3K-alpha, pi3-k, pi3k
Reactivity
Human
(93), (42), (37), (14), (12), (2), (1)
Host
Mouse
(80), (7), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(55), (48), (29), (28), (20), (6), (6), (5), (4), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 3 to 4 Business Days
Catalog No. ABIN969555
396.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of human PIK3CA expressed in E. coli.
Clone 4F3
Isotype IgG1
Purification Purified
Alternative Name PIK3CA
Background Synonyms:
PI3K, p110-α
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns (4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Molecular Weight 124 kDa
Gene ID 5290
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (2) Figure 4: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red) .
anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (3) Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye.
anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (4) Figure 1: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (5) Figure 2: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068) -hIgGFc transfected HEK293 (2) cell lysate.
Product cited in: Edwards, Witherspoon, Wang et al.: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, 2009 (PubMed).

Pang, Flinn, Patsialou et al.: "Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase." in: Cancer research, Vol. 69, Issue 23, pp. 8868-76, 2009 (PubMed).

Hosts (80), (7), (7)
Reactivities (93), (42), (37), (14), (12), (2), (1)
Applications (55), (48), (29), (28), (20), (6), (6), (5), (4), (1), (1)
Conjugates (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (14), (12), (8), (6), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN969555
396.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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