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T Antigen (AA 218-352) antibody

Details for Product No. ABIN969569, Supplier: Log in to see
AA 218-352
Clonality (Clone)
Monoclonal ()
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Immunogen Purified recombinant fragment of human T (AA 218-352) expressed in E. coli.
Clone 6C12
Isotype IgG1
Purification Purified
Background The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Molecular Weight 47.4 kDa
Gene ID 6862
HGNC 6862
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-T Antigen (AA 218-352) antibody (ABIN969569) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-T Antigen (AA 218-352) antibody (ABIN969569) Figure 2: Flow cytometric analysis of A549 cells using T mouse mAb (green) and negati...
Western Blotting (WB) image for anti-T Antigen (AA 218-352) antibody (ABIN969569) Figure 3: Western blot analysis using T mAb against human T recombinant protein. (Exp...
Western Blotting (WB) image for anti-T Antigen (AA 218-352) antibody (ABIN969569) Figure 4: Western blot analysis using T mAb against HEK293 (1) and T (AA: 218-352) -h...
Product cited in: Shen, Li, Yang, Lu, Zou, Wang, Deng: "Classic chordoma coexisting with benign notochordal cell rest demonstrating different immunohistological expression patterns of brachyury and galectin-3." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 1, pp. 96-9, 2010 (PubMed).

Tirabosco, Mangham, Rosenberg, Vujovic, Bousdras, Pizzolitto, De Maglio, den Bakker, Di Francesco, Kalil, Athanasou, ODonnell, McCarthy, Flanagan: "Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue." in: The American journal of surgical pathology, Vol. 32, Issue 4, pp. 572-80, 2008 (PubMed).