HLA-DR-gamma (CD74) antibody

Antigen: HLA-DR-gamma (CD74)

Synonyms: DHLAG, HLADG, Ia-GAMMA, Ii, CLIP

Clonality: Monoclonal (LN2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96994

Additional Information

Alternative name: CD74

Immunogen: SU-DHL-4 lymphoma cells.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: LN2

Specificity: This antibody is specific to CD74. This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with CD74, B cell antibody

Publications

Publications: Okon, Felder, Epstein et al.: "Monoclonal antibodies reactive with B-lymphocytes and histiocytes in paraffin sections." in: Cancer, Vol. 56, Issue 1, pp. 95-104, 1985 (PubMed).

Sherrod, Felder, Levy et al.: "Immunohistologic identification of phenotypic antigens associated with Hodgkin and Reed-Sternberg cells. A paraffin section study." in: Cancer, Vol. 57, Issue 11, pp. 2135-40, 1986 (PubMed).

Marder, Variakojis, Silver et al.: "Immunohistochemical analysis of human lymphomas with monoclonal antibodies to B cell and Ia antigens reactive in paraffin sections." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 52, Issue 5, pp. 497-504, 1985 (PubMed).

Epstein, Marder, Winter et al.: "Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 1028-36, 1984 (PubMed).