HLA-DR-gamma (CD74) antibody

Antigen: HLA-DR-gamma (CD74)

Synonyms: DHLAG, HLADG, Ia-GAMMA, Ii, CLIP

Clonality: Monoclonal (LN2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96995

Additional Information

Alternative name: CD74

Format: Supernatant (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: LN2

Specificity: This antibody reacts with a 35 kD antigen present on the nuclear membrane protein related to the invariant chain of the HLA-Dr antigen. This antibody reacts with the germinal center and mantle zone B lymphocytes, interdigitating histiocytes in T cell zones and mononuclear cells and Reed-Sternberg cells in Hodgkin's disease. This antibody may be useful in the identification of germinal center derived B cell lymphomas and leukemias.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Okon, Felder, Epstein et al.: "Monoclonal antibodies reactive with B-lymphocytes and histiocytes in paraffin sections." in: Cancer, Vol. 56, Issue 1, pp. 95-104, 1985 (PubMed).

Sherrod, Felder, Levy et al.: "Immunohistologic identification of phenotypic antigens associated with Hodgkin and Reed-Sternberg cells. A paraffin section study." in: Cancer, Vol. 57, Issue 11, pp. 2135-40, 1986 (PubMed).

Marder, Variakojis, Silver et al.: "Immunohistochemical analysis of human lymphomas with monoclonal antibodies to B cell and Ia antigens reactive in paraffin sections." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 52, Issue 5, pp. 497-504, 1985 (PubMed).

Epstein, Marder, Winter et al.: "Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 1028-36, 1984 (PubMed).