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CD95 antibody
| Antigen | CD95 |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (IHC)
|
 |
3 references available |
| Catalog no. | ABIN97060 |
| Quantity | 1ml (100ug/ml approximately) (Variants) |
| Price | 523.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | CD95 (Fas) |
| Immunogen | A synthetic peptide from the C-terminus of human Fas protein. |
| Format | Ig-fraction, Purified |
| Specificity | This antibody reacts with a 48 kD transmembrane glycoprotein, known as CD95 (Fas). The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell lines. CD95 mediates receptor-triggered apoptosis. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Small intestine. Cellular localization: Cell membrane |
| Concentration | 100ug/ml approximately |
| Buffer | Purified immunoglobulin fraction of rabbit antiserum against human CD95 containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | CD Antigens |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Publications
| Publications |
Kondo, Yoshino, Nishiuchi et al.: "Expression of Fas ligand mRNA in germinal centres of the human tonsil." in: The Journal of pathology, Vol. 183, Issue 1, pp. 75-9, 1997 (PubMed).
Sugihara, Saiki, Tsuji et al.: "Expression of Fas and Fas ligand in the testes and testicular germ cell tumors: an immunohistochemical study." in: Anticancer research, Vol. 17, Issue 5B, pp. 3861-5, 1998 (PubMed). Sasaki, Ahmed, Takeuchi et al.: "Immunohistochemical study of Fas, Fas ligand and interleukin-1 beta converting enzyme expression in human prostatic cancer." in: British journal of urology, Vol. 81, Issue 6, pp. 852-5, 1998 (PubMed). |
Alternatives
Alternatives for antigen "CD95", type "Antibodies"
| Hosts | Mouse (92), Rabbit (19), Goat (1) |
| Reactivities | Human (109), Monkey (3), Mouse (Murine) (3), Rat (Rattus) (3) |
| Applications | Flow Cytometry (FACS) (64), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (1) |
| Conjugates | FITC (10), PE (10), APC (6), Biotin (4), SPRD (2) |
| Epitopes | C-Term (4), pTyr232 (2), pTyr291 (2), Center (1) |
