CD99 (CD99) antibody

Antigen: CD99 (CD99)

Synonyms: MIC2, HBA71, MIC2X, MIC2Y, MSK5X

Clonality: Monoclonal (HO36-1-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN97071

Additional Information

Alternative name: CD99

Immunogen: BALB/C mice were injected with purified E-rosette forming cells from human peripheral blood lymphocytes.

Format: Supernatant (prediluted)

Isotype: IgM kappa  (Matching secondary antibodies)

Clone: HO36-1-1

Specificity: This antibody is specific to a sialoglycoprotein of 27-32 kD known as CD99 or MIC2 gene product. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes and granulosa cells of the ovary, sertoli cells of testis, pancreatic islet cells and some endothelial cells. Mature granulocytes express very little or no CD99.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Sandrin, Vaughan, Henning et al.: "Expression cloning of cDNA clones encoding the human cell surface proteins HuLy-m6 and FMC29." in: Immunogenetics, Vol. 35, Issue 4, pp. 283-5, 1992 (PubMed).