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Antibodies
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anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibody
Vascular Cell Adhesion Molecule 1 (VCAM1) antibody
| Antigen |
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| Synonyms |
CD106, MGC99561, INCAM-100, DKFZp779G2333, Vcam-1, VCAM1B, MGC108734, VCAM1, fi76d06, wu:fi76d06, zgc:158875, VCAM-1, DKFZp469N1820 |
| Clonality |
Monoclonal (1-G11B1) |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (159), Immunohistochemistry (Frozen Sections) (IHC (fro)) (57), Immunoprecipitation (IP) (48), Western Blotting (WB) (38), Immunofluorescence (IF) (35), ELISA (30), Immunohistochemistry (IHC) (23), Functional Studies (Func) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Enzyme Immunoassay (EIA) (11), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN97086 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
283.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD106
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Immunogen
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Raised against stimulate HUVEC cells.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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1-G11B1
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Specificity
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This antibody is specific to 110 kD membrane associated protein, identified as vascular cell adhesion molecule (VCAM-1). The ligand for VCAM-1 is VLA-4 (CD49d/CD29, alpha 4 beta 1 integrin subunits). CD106 is present on activated endothelial cells, tissue macrophages, dendrite cells and bone marrow fibroblasts. The CD106 antigen mediates melanoma adhesion to endothelial cells and may be involved in the metastatic spread of tumor.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody is recommended for frozen tissue sections only. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Fleming: "Cellular functions of adhesion molecules." in: The Journal of pathology, Vol. 161, Issue 3, pp. 189-90, 1990 (PubMed).
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Alternatives for antigen "Vascular Cell Adhesion Molecule 1 (VCAM1)", type "Antibodies"
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Hosts
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Mouse (114), Rat (62), Rabbit (26), Sheep (2)
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Reactivities
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Human (124), Mouse (Murine) (81), Rat (Rattus) (29), Monkey (5), Pig (Porcine) (5), Dog (Canine) (4), Chicken (2), Cow (Bovine) (2), Horse (Equine) (2), Rabbit (2)
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Applications
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Flow Cytometry (FACS) (159), Immunohistochemistry (Frozen Sections) (IHC (fro)) (57), Immunoprecipitation (IP) (48), Western Blotting (WB) (38), Immunofluorescence (IF) (35), ELISA (30), Immunohistochemistry (IHC) (23), Functional Studies (Func) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Enzyme Immunoassay (EIA) (11), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (30), FITC (27), PE (17), RPE (4), APC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
