»
Antibodies
»
anti-Cyclin-Dependent Kinase 2 (CDK2) antibody
Cyclin-Dependent Kinase 2 (CDK2) antibody
| Antigen |
|
| Synonyms |
p33(CDK2), A630093N05Rik, MGC849, 1810047K05Rik, 2810452K22Rik, RGD1563395, CINP, 2810452K22RIK, MGC84068, cinp, MGC166364, zgc:56598, zgc:77684, wu:fa10c02, CDK2 |
| Clonality |
Monoclonal (2B6) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
Western Blotting (WB) (103), Immunofluorescence (IF) (53), Flow Cytometry (FACS) (38), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), ELISA (22), Immunohistochemistry (IHC) (18), Enzyme Immunoassay (EIA) (17), Immunoprecipitation (IP) (12), Dot Blot (Dot) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN97129 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
482.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
cdk2
|
|
Immunogen
|
BALB/C mice were injected with a human recombinant cdk2 protein.
|
|
Format
|
Ig-fraction
|
|
Isotype
|
IgG2b (Matching secondary antibodies)
|
|
Clone
|
2B6
|
|
Specificity
|
This antibody reacts with a 33 kDa protein known as cdk2. cdk-2 complexes with cyclins E, D1 and D3. Cyclin E-cdk2 kinase is active in the G1 and S- phases of the cell cycle and is important (as does Cyclin A-cdk2) for the progression from G1 to S-phase. The levels of Cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and Cyclin A are found associated with DNA in the initiation complex during replication. This antibody cross reacts with mouse and rat.
|
|
Application Notes
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil. Cellular localization: Cytoplasmic, nuclear Other Applications: Western Blotting (1:100-500).
|
|
Concentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
Cell Cycle
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
|
Publications
|
Bresnahan, Thompson, Albrecht: "Human cytomegalovirus infection results in altered Cdk2 subcellular localization." in: The Journal of general virology, Vol. 78 ( Pt 8), pp. 1993-7, 1997 (PubMed).
|
Alternatives for antigen "Cyclin-Dependent Kinase 2 (CDK2)", type "Antibodies"
|
Hosts
|
Rabbit (80), Mouse (44)
|
|
Reactivities
|
Human (107), Mouse (Murine) (46), Rat (Rattus) (43), Monkey (3), Xenopus laevis (2), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1)
|
|
Applications
|
Western Blotting (WB) (103), Immunofluorescence (IF) (53), Flow Cytometry (FACS) (38), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), ELISA (22), Immunohistochemistry (IHC) (18), Enzyme Immunoassay (EIA) (17), Immunoprecipitation (IP) (12), Dot Blot (Dot) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
pThr160 (14), C-Term (10), Center R199 (1), T14 (1), T160 (1), pT14 (1), pT41 (1)
|
Alternatives 
