Cyclin-Dependent Kinase 9 (CDK9) antibody

Antigen: Cyclin-Dependent Kinase 9 (CDK9)

Synonyms: TAK, C-2k, CTK1, CDC2L4, PITALRE, CDK9, P-TEF, P-TEFb, PTefb, DmelCG5179, CG5179, wu:fb22e09, wu:fc06c02, MGC95175, cdk9, MGC53831, MGC139578, tak, c-2k, cdc2l4, pitalre, MGC69296

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN97135

Additional Information

Alternative name: cdk9 (Pitalre)

Gene ID: NP_001252

Swiss-Prot: P50750

Immunogen: Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE). Protein Sequence: Human CDK9 (PITALRE), 372 aa, predicted MW 42.8 kDa 1 makqydsvec pfcdevskye klakigqgtf gevfkarhrk tgqkvalkkv lmenekegfp 61 italreikil qllkhenvvn lieicrtkas pynrckgsiy lvfdfcehdl agllsnvlvk 121 ftlseikrvm qmllnglyyi hrnkilhrdm kaanvlitrd gvlkladfgl arafslakns 181 qpnrytnrvv tlwyrppell lgerdygppi dlwgagcima emwtrspimq gnteqhqlal 241 isqlcgsitp evwpnvdnye lyeklelvkg qkrkvkdrlk ayvrdpyald lidkllvldp 301 aqridsddal nhdffwsdpm psdlkgmlst hltsmfeyla pprrkgsqit qqstnqsrnp 361 attnqtefer vf

Format: Liquid (sterile filtered)

Description: CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.

Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 43 kDa cdk9 (PITALRE) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk9 (PITALRE) from other species may also occur. The murine cDNA is shown to be 98% identical with human.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000 OTHER APPLICATIONS User Optimized . Note: This antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and by western blot. For immunohistochemistry use paraffin embedded tissue. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa corresponding to CDK9 (PITALRE) by western blotting in the appropriate cell lysate or extract.

Concentration: 75 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Other detection systems should yield similar results. Sections from each specimen were cut at 5-7 uM, mounted on glass and dried overnight at 37

anti-Cyclin-Dependent Kinase 9 (CDK9) antibody (Image 2)

anti-Cyclin-Dependent Kinase 9 (CDK9) antibody (Image 3)