Cecropin B antibody

Antigen: Cecropin B

Synonyms: Cec, cecB, DmelCG1878, CG1878, Cecb1, Cecb, dsim_GLEANR_1734, DsimGD17190, GD17190, CecB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN97136

Additional Information

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic full-length cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu.

Format: Liquid (sterile filtered)

Description: Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.

Application Details

Application Notes: Recommended Dilutions: This reagent is suitable for the detection of cecropin B in immunoblotting, immunoprecipitation and ELISA experiments. A dilution of 1:1000 is recommended for immunoblotting experiments. A single 4400 dalton band is detected in protein extracts. Researchers should determine optimal titers for other applications. Note: Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value for the study of cecropins in transgenic plants. The antibody may also recognize naturally occurring cecropin A. Cecropin B has been modified in some instances by substituting a single amino acid to render the molecule less susceptible to proteolytic cleavage.

Concentration: 0.33 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 50% (v/v) Glycerol. Preservative: 0.005% (w/v) Sodium Azide. Buffer: 0.01 M Potassium Phosphate, 0.075 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. General

Restrictions: For Research Use only