c-erb-2 antibody

Antigen: c-erb-2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN97149

Additional Information

Immunogen: Keyhole limpet hemocyanin conjugated synthetic human peptide c-erbB-2 oncoprotein.

Format: Antiserum

Specificity: This antibody reacts with the c-erbB-2 oncoprotein, a 190 kD protein. The proto-oncogene for c-erbB-2 is located on the human chromosome 17, band 21. It has structural similarities to EGFR. Several studies have indicated that c-erbB-2 may be a good indicator of the prognosis of human carcinomas in the breast, ovary, uterus and gastrointestinal tract.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Although not required, high temperature treatment may enhance the immunostaining in overfixed tissues. Positive Control: Breast carcinoma. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: Affinity purified rabbit antiserum against human c-erbB-2 oncoprotein containing azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Receptors, Growth Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with c-erbB-2 antibody

Publications

Publications: Fukushige, Matsubara, Yoshida et al.: "Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 955-8, 1986 (PubMed).

Wright, Angus, Nicholson et al.: "Expression of c-erbB-2 oncoprotein: a prognostic indicator in human breast cancer." in: Cancer research, Vol. 49, Issue 8, pp. 2087-90, 1989 (PubMed).

Schechter, Hung, Vaidyanathan et al.: "The neu gene: an erbB-homologous gene distinct from and unlinked to the gene encoding the EGF receptor." in: Science (New York, N.Y.), Vol. 229, Issue 4717, pp. 976-8, 1985 (PubMed).

Wu, Zhang, Lyons et al.: "Isolation of the intact molecule and ectodomain of C-erbB-2 oncoprotein from SK-BR-3 cells and development of immunoassays on microplate." in: Journal of clinical laboratory analysis, Vol. 12, Issue 5, pp. 298-303, 1998 (PubMed).