Chromogranin A (CHGA) antibody

Antigen: Chromogranin A (CHGA)

Synonyms: CGA, MGC105435, fj05f09, wu:fj05f09, zgc:101749, si:dkey-177p2.2, CHGA

Clonality: Monoclonal (LK2H10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN97321

Additional Information

Alternative name: Chromogranin A

Immunogen: BALB/C mice were immunized with human phaeochromocytoma.

Format: Supernatant

Isotype: IgG1 kappa

Clone: LK2H10

Specificity: Chromogranin A is a 439 amino acid protein present in secretory granules of a wide variety of endocrine cells and neurons. A positive staining is seen in the secretory granules of parathyroid, adrenal medulla, anterior pituitary gland and Langerhans islets of pancreas. This antibody stains a variety of neuroendocrine tumors.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Pancreas. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Neurotransmitters

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human pancreas stained with Chromogranin A antibody

Publications

Burke, Federspiel, Sobin et al.: "Carcinoids of the duodenum. A histologic and immunohistochemical study of 65 tumors." in: The American journal of surgical pathology, Vol. 13, Issue 10, pp. 828-37, 1989 (PubMed).

Weiler, Fischer-Colbrie, Schmid et al.: "Immunological studies on the occurrence and properties of chromogranin A and B and secretogranin II in endocrine tumors." in: The American journal of surgical pathology, Vol. 12, Issue 11, pp. 877-84, 1988 (PubMed).

Lloyd, Cano, Rosa et al.: "Distribution of chromogranin A and secretogranin I (chromogranin B) in neuroendocrine cells and tumors." in: The American journal of pathology, Vol. 130, Issue 2, pp. 296-304, 1988 (PubMed).