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Chromogranin A (CHGA) antibody

Antigen

Chromogranin A (CHGA)
Synonyms CGA, MGC105435, fj05f09, wu:fj05f09, zgc:101749, si:dkey-177p2.2, CHGA
Clonality Monoclonal (LK2H10)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN97322
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 335.50 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Chromogranin A
Immunogen Purified C-terminal half of chromogranin A from urine of patients with carcinoid syndrome.
Format Supernatant (prediluted)
Isotype IgG1 kappa
Clone LK2H10
Specificity Chromogranin A is a 439 amino acid protein present in secretory granules of a wide variety of endocrine cells and neurons. A positive staining is seen in the secretory granules of parathyroid, adrenal medulla, anterior pituitary gland and Langerhans islets of pancreas. This antibody stains a variety of neuroendocrine tumors.

Application Details
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Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Pancreas. Cellular localization: Cytoplasmic
Concentration 1 to 4 ug/ml
Purification Supernatant (prediluted)
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Cancer, Neurotransmitters
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
Application Details Alternatives Variants back to top

Burke, Federspiel, Sobin et al.: "Carcinoids of the duodenum. A histologic and immunohistochemical study of 65 tumors." in: The American journal of surgical pathology, Vol. 13, Issue 10, pp. 828-37, 1989 (PubMed).

Weiler, Fischer-Colbrie, Schmid et al.: "Immunological studies on the occurrence and properties of chromogranin A and B and secretogranin II in endocrine tumors." in: The American journal of surgical pathology, Vol. 12, Issue 11, pp. 877-84, 1988 (PubMed).

Lloyd, Cano, Rosa et al.: "Distribution of chromogranin A and secretogranin I (chromogranin B) in neuroendocrine cells and tumors." in: The American journal of pathology, Vol. 130, Issue 2, pp. 296-304, 1988 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Chromogranin A (CHGA)", type "Antibodies"
Hosts Mouse (69), Rabbit (67), Goat (6), Sheep (4), Chicken (1), Guinea Pig (1)
Reactivities Human (138), Rat (Rattus) (31), Mouse (Murine) (26), Pig (Porcine) (7), Monkey (6), Rabbit (5), Cow (Bovine) (4), Dog (Canine) (2), Cat (Feline) (1), Chicken (1)
Applications Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (73), Western Blotting (WB) (65), ELISA (38), Immunohistochemistry (IHC) (29), Immunofluorescence (IF) (28), Immunohistochemistry (Fixed) (IHC (fx)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (7), Flow Cytometry (FACS) (5), Immunoprecipitation (IP) (4), Immunocytochemistry (ICC) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Cell-ELISA (cELISA) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (5), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (4), Center (4), AA 19-131 (3), N-Term (3), AA 1-15 (2), Internal Region (1), Precursor (1)