V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) antibody

Antigen: V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyms: MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746

Clonality: Monoclonal (9E10)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN97439

Additional Information

Alternative name: c-Myc

Immunogen: Synthetic peptide corresponding to residues 408-439 of the human p62c-myc protein.

Format: Ascites

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9E10

Specificity: This antibody recognizes an epitope located in the amino acid residue 410-419 of human oncogene product c-myc. This antibody reacts with both components of the p62c-myc-p64c-myc. This antibody cross reacts with human p62/64c-myc but does not react with mouse p62/64c-myc protein.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Breast carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western blotting (1:100-500).

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Royds, Sharrard, Wagner et al.: "Cellular localisation of c-myc product in human colorectal epithelial neoplasia." in: The Journal of pathology, Vol. 166, Issue 3, pp. 225-33, 1992 (PubMed).

Campbell, Kessler, Fambrough: "The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9321-5, 1992 (PubMed).

Williams, Piris, Wyllie: "Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms." in: The Journal of pathology, Vol. 160, Issue 4, pp. 287-93, 1990 (PubMed).

Evan, Lewis, Ramsay et al.: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986 (PubMed).