Collagen, Type IV, alpha 1 (COL4A1) antibody

Antigen: Collagen, Type IV, alpha 1 (COL4A1)

Synonyms: arresten, Bru, Raw, Svc, Col4a-1, Del(8)44H, col4a1, wu:fc02g11, COL4A1

Clonality: Monoclonal (COL-94)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN97504

Additional Information

Alternative name: Collagen Type IV

Immunogen: Human collagen type IV.

Format: Ascites

Isotype: IgG1  (Matching secondary antibodies)

Clone: COL-94

Specificity: This antibody recognizes collagen type IV epitope located on the alpha1 and/or alpha 2 chains of human collagen type IV. This antibody shows no cross reactivity with collagen types I, II, III, V, VI and VII as well as does not react with human vitronectin, fibronectin or chondroitin sulfate A, B and C.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment prior to immunostaining is required for formalin fixed tissue sections. Positive Control: Skin. Cellular localization: Basement membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Sage, Woodbury, Bornstein: "Structural studies on human type IV collagen." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9893-900, 1979 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).