Cullin 1 (CUL1) antibody
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| Synonyms |
LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN97899 |
| Quantity |
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| Price |
598.40 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 3 to 4 Business Days |
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Alternative name
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Cullin-1 (CUL-1)
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Immunogen
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A peptide derived from the C-terminus region of the human Cullin-1.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 86kD protein, known as Cullin-1. Cullin-1 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b and 5) homologous to the S. cerevisiae cdc53 gene. CUL-1 forms a complex with human p19skp1 and F box protein p45skp2, together with cdc53, as three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. This antibody cross reacts with mouse.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western Blotting
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Concentration
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100ug/ml approximately
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Purification
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Ig-fraction,Purified
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against human Cullin-1 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Apoptosis/Necrosis, Proteasome
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).
Pagano: "Cell cycle regulation by the ubiquitin pathway." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 11, Issue 13, pp. 1067-75, 1997 (PubMed).
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Alternatives for antigen "Cullin 1 (CUL1)", type "Antibodies"
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Hosts
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Rabbit (51), Mouse (3)
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Reactivities
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Human (53), Mouse (Murine) (33), Rat (Rattus) (26), Cow (Bovine) (19), Pig (Porcine) (18), Chicken (2), Dog (Canine) (2), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)
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Applications
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Western Blotting (WB) (39), ELISA (23), Immunofluorescence (IF) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunoprecipitation (IP) (15), Immunohistochemistry (IHC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (9), AA 766-776 (1), AA 766-776,C-Term (1), C-Term AA 766-776 (1), N-Term (1)
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2013 BioMed Central Research Awards
