Cyclin D1 (CCND1) antibody

Details for Product No. ABIN97954
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Antigen
Synonyms
ccnd1b, prad1, bcl1, d11s287e, u21b31, ccndbp1, ccnd1, ZHM, CCND1, BCL1, D11S287E, PRAD1, U21B31, AI327039, CycD1, Cyl-1, bcl-1, cD1, ccnd1a, cb161, cycd1, etID37810.7, fb52e01, fc45c08, fc83a12, id:i ... show more
ccnd1b, prad1, bcl1, d11s287e, u21b31, ccndbp1, ccnd1, ZHM, CCND1, BCL1, D11S287E, PRAD1, U21B31, AI327039, CycD1, Cyl-1, bcl-1, cD1, ccnd1a, cb161, cycd1, etID37810.7, fb52e01, fc45c08, fc83a12, id:ibd1198, wu:fb52e01, wu:fc45c08, wu:fc83a12 show less
Reactivity
Human
(263), (102), (99), (32), (19), (10), (9), (4), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(229), (72), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(12), (10), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(244), (90), (80), (56), (55), (40), (35), (33), (20), (19), (11), (3), (3), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN97954
361.90 $
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Immunogen Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name Cyclin D1
Background Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family.  Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Synonyms: G1/S-specific cyclin-D1 PRAD1 oncogene BCL-1 oncogene
Gene ID 595, 16950655
UniProt P24385
Research Area Cancer, Cell Cycle
Application Notes This antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. MCF7 may be used as a positive control. Anti-Cyclin D1is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Cyclin D1 (CCND1) antibody Western blot analysis is shown using anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG 611-103-122 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results.
General Kenerson, Aicher, True et al.: "Activated mammalian target of rapamycin pathway in the pathogenesis of tuberous sclerosis complex renal tumors." in: Cancer research, Vol. 62, Issue 20, pp. 5645-50, 2002 (PubMed).

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