Cyclin D1 (CCND1) antibody

Antigen: Cyclin D1 (CCND1)

Synonyms: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN97954

Additional Information

Alternative name: Cyclin D1

Gene ID: NP_444284

Swiss-Prot: P24385

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length syntheticpolypeptide corresponding to the human gene sequence.

Format: Liquid (sterile filtered)

Description: Cyclin D 1 (also known as G 1 /S-specific cyclin D 1 , PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. Cyclins are characterized by adramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibitdistinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D 1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G 1 /S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety oftumors and may contribute to tumorigenesis.

Specificity: This is an antiserum produced by delipidation an d defibrination. Reactivity occurs against human Cyclin D 1 protein. Expect cross reactivity with Cyclin D 1 from many other organisms as the protein, or domains within the protein, are highly homologous in human, chimpanzee, orangutan, rat, mouse, dog, chicken, frog, and zebrafish. Reactivity with Cyclin D 1 proteins from other sources is not known.

Synonyms: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Application Details

Application Notes: This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to Cyclin D 1 by western blotting in the appropriate cell lysate or extract. Anti-Cyclin D 1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin D 1. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Diluteonly prior to immediate use. For extended storage aliquot contentsand freeze at -20° C or below. Avoid cycles of freezing andthawing. Expiration date is one (1) year from date of opening.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG ABIN102010 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results. Personal communication Luca Cote.

A model summarizes the interactions between cyclins and the cyclin dependent kinases in multicellular animal cells. The R point is the restriction point. The diagram shows the stages of the cell cycle and the binding of the specified cyclins with the corresponding CDKs at each stage. cdc2 is kinase, p107 and E2F are proteins involved in transcription.

Publications

Publications: Kenerson, Aicher, True et al.: "Activated mammalian target of rapamycin pathway in the pathogenesis of tuberous sclerosis complex renal tumors." in: Cancer research, Vol. 62, Issue 20, pp. 5645-50, 2002 (PubMed).