Cyclin E1 (CCNE1) antibody

Antigen: Cyclin E1 (CCNE1)

Synonyms: CCNE, AW538188, Ccne, CYCLE, cycE1, cyce2, cycE3, MGC85048, CCNE1, ccne1

Clonality: Monoclonal (HE12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN97972

Additional Information

Alternative name: Cyclin E

Immunogen: Synthetic peptide corresponding to human cyclin E protein.

Format: Supernatant

Isotype: IgG1

Clone: HE12

Specificity: This antibody recognizes a doublet of 50 kD and a single band of 42 kD identified as cyclin E. Cyclin proteins are a family of related proteins, which control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E antibody

Publications

Duli?, Lees, Reed: "Association of human cyclin E with a periodic G1-S phase protein kinase." in: Science (New York, N.Y.), Vol. 257, Issue 5078, pp. 1958-61, 1992 (PubMed).

Koff, Cross, Fisher et al.: "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family." in: Cell, Vol. 66, Issue 6, pp. 1217-28, 1991 (PubMed).

Ohtsubo, Theodoras, Schumacher et al.: "Human cyclin E, a nuclear protein essential for the G1-to-S phase transition." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2612-24, 1995 (PubMed).

Sewing, Roenicke, Buerger et al.: "Alternative splicing of human cyclin E." in: Journal of cell science, Vol. 107 ( Pt 2), pp. 581-8, 1994 (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, 1993 (PubMed).