Cyclin E (CCNE) antibody

Antigen: Cyclin E (CCNE)

Synonyms: Cyce, Ccne, CYCLE, cyce, fa18e07, wu:fa18e07, BG:DS07108.3, CDI7, CYCE, Cyc E, CyclE, CyeE, D-CycE, DmCycE, DmcyclinE, br37, cyclinE, dm-cycE, fond, l(2)05206, l(2)35Dd, l(2)br37, l(2)k02514, l(2)k02602, l(2)k05007, l35Dd, DmelCG3938, CG3938, NV18076

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN97973

Additional Information

Alternative name: Cyclin E

Immunogen: Full length synthetic polypeptide corresponding to the human gene sequence.

Format: Liquid (sterile filtered)

Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 45 kDa Cyclin E protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin E from other species may also occur.

Synonyms: Cyce, Ccne, CYCLE, cyce, fa18e07, wu:fa18e07, BG:DS07108.3, CDI7, CYCE, Cyc E, CyclE, CyeE, D-CycE, DmCycE, DmcyclinE, br37, cyclinE, dm-cycE, fond, l(2)05206, l(2)35Dd, l(2)br37, l(2)k02514, l(2)k02602, l(2)k05007, l35Dd, DmelCG3938, CG3938, NV18076

Application Details

Application Notes: Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Cyclin E at a dilution of 1:200 to 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code ABIN101990. Anti-Cyclin E is suitable for the detection by immunoblot of human, rat and mouse Cyclin E. Suggested dilution for ELISA is 1:2,000. Optimal titers for other applications should be determined by the researcher. suitable for ELISA, immunoprecipitation, immunoblotting and other immunological methods requiring high titer and specificity.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Publications: Simone, Resta, Bagella et al.: "Cyclin E and chromosome instability in colorectal cancer cell lines." in: Molecular pathology : MP, Vol. 55, Issue 3, pp. 200-3, 2002 (PubMed).