Cystatin A (Stefin A) (CSTA) antibody

Antigen: Cystatin A (Stefin A) (CSTA)

Synonyms: STF1, STFA, CSTA, CST, CYSTATIN A, FL3-27, T20B5.8, T20B5_8

Clonality: Monoclonal (WR-23-2-2-3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98003

Additional Information

Alternative name: Cystatin A

Immunogen: Human Cystatin A.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: WR-23-2-2-3

Specificity: This antibody reacts with 11-13 kD protein, Cystatin A. Cystatins are endogenous inhibitors of lysosomal proteases that form a superfamily, which is classified into 1, 2, and 3 families. Cystatin A is thought to be a tumor suppressor, since a inverse correlation seems to exist between the level of Cystatin A and tumor progression. Cystatin A has been implicated in aging of tissues, Alzheimer disease, viral infection and inflammatory processes.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Pretreatment with trypsin is required for paraffin sections. Positive Control: Brain. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Serum/Plasma Proteins

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Eide, Jaervinen, Hopsu-Havu et al.: "Immunolocalization of cystatin A in neoplastic, virus and inflammatory lesions of the uterine cervix." in: Acta histochemica, Vol. 93, Issue 1, pp. 241-8, 1992 (PubMed).

Takeda, Iwasawa, Nakamura et al.: "Monoclonal antibodies as probes to detect conformational changes in the rat cysteine proteinase inhibitor cystatin A." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 69-78, 1994 (PubMed).

Bernstein, Rinne, Kirschke et al.: "Cystatin A-like immunoreactivity is widely distributed in human brain and accumulates in neuritic plaques of Alzheimer disease subjects." in: Brain research bulletin, Vol. 33, Issue 5, pp. 477-81, 1994 (PubMed).

Kuopio, Kankaanranta, Jalava et al.: "Cysteine proteinase inhibitor cystatin A in breast cancer." in: Cancer research, Vol. 58, Issue 3, pp. 432-6, 1998 (PubMed).