Cytokeratin 18 (KRT18) antibody

Antigen: Cytokeratin 18 (KRT18)

Synonyms: K18, CK18, Krt1-18, CYK18

Clonality: Monoclonal (AE3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98101

Additional Information

Alternative name: Cytokeratin 1-8

Immunogen: BALB/C mice were immunized with human epidermal keratin.

Format: Ig-fraction

Isotype: IgG1-kappa  (Matching secondary antibodies)

Clone: AE3

Specificity: This antibody recognizes most of the basic (Type II) keratins. It recognizes keratin polypeptides of 58 and 65 to 67 kD. In keratinized epidermis, 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. This antibody can be used to stain different cell layers in an epithelial tissue and various neoplasms of epithelial origin.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasm

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified IgG fraction harvested from ascites and contains sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Klein-Szanto, Boysen, Reith: "Keratin and involucrin in preneoplastic and neoplastic lesions. Distribution in the nasal mucosa of nickel workers." in: Archives of pathology & laboratory medicine, Vol. 111, Issue 11, pp. 1057-61, 1987 (PubMed).

Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).