Keratin1-19 antibody

Antigen: Keratin1-19

Clonality: Monoclonal (AE1-AE3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98105

Additional Information

Alternative name: Cytokeratin 1-19

Immunogen: BALB/C mice were immunized with human epidermal keratin.

Format: Supernatant

Isotype: IgG1-kappa  (Matching secondary antibodies)

Clone: AE1-AE3

Specificity: This AE1/AE3 antibody cocktail recognizes most of the acidic (Type I) keratins as well as basic (Type II). It recognizes keratin polypeptides of 40, 48, 50, 52, 54, 56.5, 58, 59 and 64 to 67 kD. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. This antibody can be used to stain distinct cell layers in an epithelial tissue and various neoplasms of epithelial origin.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified IgG fraction harvested from ascites and contains sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Cytokeratin antibody cocktail AE1 + AE3

Publications

Publications: Spagnolo, Michie, Crabtree et al.: "Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms." in: American journal of clinical pathology, Vol. 84, Issue 6, pp. 697-704, 1986 (PubMed).

Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).

Sun, Eichner, Nelson et al.: "Keratin classes: molecular markers for different types of epithelial differentiation." in: The Journal of investigative dermatology, Vol. 81, Issue 1 Suppl, pp. 109s-15s, 1983 (PubMed).