Cytokeratin 4 (KRT4) antibody

Antigen: Cytokeratin 4 (KRT4)

Synonyms: K4, CK4, CYK4, FLJ31692

Clonality: Monoclonal (6B10)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98112

Additional Information

Alternative name: Cytokeratin 4

Immunogen: Cytokeratin purified from human esophagus.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6B10

Specificity: This antibody is specific to 59 kD protein. Cytokeratin 4 belongs to the type B (basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 13. Cytokeratin 4 is primarily found in the non-cornifying squamous epithelium, i.e. superficial and intermediate epithelial cells of esophagus, ectocervix, tongue, vagina, larynx, pharynx, epiglottis, anus and cornea. Cytokeratin 4 may be detected in squamous cell carcinomas derived from several non-cornified epithelia.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Cytokeratin 4 antibody

Publications

Publications: Omoto, Miyashita, Shimmura et al.: "The use of human mesenchymal stem cell-derived feeder cells for the cultivation of transplantable epithelial sheets." in: Investigative ophthalmology & visual science, Vol. 50, Issue 5, pp. 2109-15, 2009 (PubMed).