Keratin 5/6 (KRT5/6) antibody

Antigen: Keratin 5/6 (KRT5/6)

Clonality: Monoclonal (D5-16 B4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98116

Additional Information

Alternative name: Cytokeratin 5 & 6

Immunogen: BALB/C mice were injected with cytokeratin 5.

Format: Ig-fraction (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: D5-16 B4

Specificity: Cytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, the intermediate and the superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight, basic type cytokeratin expressed by proliferating squamous epithelium often paired with cytokeratin 16.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Clover, Oates, Edwards: "Anti-cytokeratin 5/6: a positive marker for epithelioid mesothelioma." in: Histopathology, Vol. 31, Issue 2, pp. 140-3, 1997 (PubMed).

Cury, Butcher, Fisher et al.: "Value of the mesothelium-associated antibodies thrombomodulin, cytokeratin 5/6, calretinin, and CD44H in distinguishing epithelioid pleural mesothelioma from adenocarcinoma metastatic to the pleura." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 13, Issue 2, pp. 107-12, 2000 (PubMed).

Otterbach, Buankfalvi, Bergner et al.: "Cytokeratin 5/6 immunohistochemistry assists the differential diagnosis of atypical proliferations of the breast." in: Histopathology, Vol. 37, Issue 3, pp. 232-40, 2000 (PubMed).