Cytokeratin 5/8 antibody

Antigen: Cytokeratin 5/8

Clonality: Monoclonal (C50)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98117

Additional Information

Alternative name: Cytokeratin 5 & 8

Immunogen: Cytoskeletal preparation for Hela cells.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: C50

Specificity: This antibody is specific to human cytokeratins 5 (58 kD) and 8 (52.5 kD). Cytokeratin 5 is expressed by basal cells and suprabasal cells of stratified epithelium. Cytokeratin 8 is expressed by simple epithelial cells. This antibody can be useful for the detection of wide range of tumors derived from epithelia.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin 5/8 antibod

Publications

Publications: Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Angus, Purvis, Stock et al.: "NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue." in: The Journal of pathology, Vol. 153, Issue 4, pp. 377-84, 1988 (PubMed).