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Antibody
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anti-Cytokeratin 8 KRT8
Cytokeratin 8 (KRT8) antibody
| Antigen |
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| Synonyms |
K8, KO, CK8, CYK8, K2C8, CARD2, K18, CYK18 |
| Clonality |
Monoclonal (35bH11) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (43), Immunofluorescence (IF) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunohistochemistry (IHC) (14), Enzyme Immunoassay (EIA) (9), Immunoprecipitation (IP) (8), ELISA (Detection) (5), ELISA (4), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (2)
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| Catalog no. |
ABIN98131 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
328.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business days |
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Alternative name
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Cytokeratin 8
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Immunogen
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BALB/C mice were immunized with cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B.
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Format
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Supernatant
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Isotype
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IgM-kappa (Matching secondary antibodies)
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Clone
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35bH11
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Specificity
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This antibody recognizes keratin polypeptide of 54 kD. It stains strongly with non-squamous epithelium but does not stain squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinomas of the ovary, gastrointestinal tract, ductal carcinoma of breast, pancreas and bile ducts, stain positive with this antibody.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Gown, Vogel: "Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors." in: American journal of clinical pathology, Vol. 84, Issue 4, pp. 413-24, 1985 (PubMed).
Gown, Vogel: "Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies." in: The Journal of cell biology, Vol. 95, Issue 2 Pt 1, pp. 414-24, 1983 (PubMed).
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Alternatives for antigen "Cytokeratin 8 (KRT8)", type "Antibodies"
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Hosts
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Mouse (46), Rabbit (38), Guinea Pig (1)
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Reactivities
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Human (71), Mouse (Murine) (14), Rat (Rattus) (12), Cow (Bovine) (8), Pig (Porcine) (8), Sheep (Ovine) (8), Rabbit (6), None (3), Hamster (2), Mammalian (1)
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Applications
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Western Blotting (WB) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (43), Immunofluorescence (IF) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunohistochemistry (IHC) (14), Enzyme Immunoassay (EIA) (9), Immunoprecipitation (IP) (8), ELISA (Detection) (5), ELISA (4), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (2)
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Conjugates
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Biotin (2), FITC (1), HRP (1)
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Epitopes
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C-Term (4), pSer74 (4), pSer73 (1)
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