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Antibody
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anti-Cytokeratin 8-18
Cytokeratin 8/18 antibody
| Antigen |
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| Clonality |
Monoclonal (5D3) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN98137 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
276.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business days |
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Alternative name
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Cytokeratin 8 & 18
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Immunogen
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Cytokeratins from the human breast carcinoma cell line MCF-7.
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Format
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Supernatant
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Isotype
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IgG1-kappa (Matching secondary antibodies)
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Clone
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5D3
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Specificity
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This antibody is specific to human cytokeratins 8 (52.5 kD) and 18 (45 kD). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Angus, Purvis, Stock et al.: "NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue." in: The Journal of pathology, Vol. 153, Issue 4, pp. 377-84, 1988 (PubMed).
Angus, Kiberu, Purvis et al.: "Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1." in: The Journal of pathology, Vol. 155, Issue 1, pp. 71-5, 1988 (PubMed).
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Alternatives for antigen "Cytokeratin 8/18", type "Antibodies"
