Cytokeratin 9-19 antibody

Antigen: Cytokeratin 9-19

Clonality: Monoclonal (AE1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98142

Additional Information

Immunogen: BALB/C mice were immunized with human epidermal keratin.

Format: Ig-fraction (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: AE1

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. This antibody recognizes most of the acidic (Type I) keratins. It recognizes keratin polypeptides of 50 and 56.5 kD. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers. This antibody can be used to stain distinct cell layers in an epithelial tissue and various neoplasms of epithelial origin. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Spagnolo, Michie, Crabtree et al.: "Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms." in: American journal of clinical pathology, Vol. 84, Issue 6, pp. 697-704, 1986 (PubMed).

Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).

Sun, Eichner, Nelson et al.: "Keratin classes: molecular markers for different types of epithelial differentiation." in: The Journal of investigative dermatology, Vol. 81, Issue 1 Suppl, pp. 109s-15s, 1983 (PubMed).