Cytokeratin 10 (KRT10) antibody

Antigen: Cytokeratin 10 (KRT10)

Synonyms: K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik

Clonality: Monoclonal (DE-K10)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98145

Additional Information

Alternative name: Cytokeratin 10

Immunogen: BALB/C mice were immunized with detergent insoluble extract of human epidermis.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: DE-K10

Specificity: This antibody recognizes keratin polypeptide of 56.5 kD. It stains suprabasal cells in the epidermis, thymic Hassal's bodies. Simple and glandular epithelia, transitional epithelium and adenocarcinomas are negative with this antibody.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is not recommended prior to the immunostaining, as it may reduce the staining. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human skin stained with Cytokeratin 10 antibody

Publications

Publications: Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

Ivanyi, Ansink, Mooi et al.: "Absence of differentiation-related expression of keratin 10 in early stages of vulvar squamous carcinoma." in: Differentiation; research in biological diversity, Vol. 42, Issue 2, pp. 124-9, 1990 (PubMed).

Chamcheu, Loriue, Akgul et al.: "Characterization of immortalized human epidermolysis bullosa simplex (KRT5) cell lines: trimethylamine N-oxide protects the keratin cytoskeleton against disruptive stress condition." in: Journal of dermatological science, Vol. 53, Issue 3, pp. 198-206, 2009 (PubMed).