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Antibodies
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anti-Cytokeratin 10 (KRT10) antibody
Cytokeratin 10 (KRT10) antibody
| Antigen |
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| Synonyms |
K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik |
| Clonality |
Monoclonal (LHP1) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN98147 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
361.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cytokeratin 10
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Immunogen
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Crude cytoskeletal extract prepared from the epidermal component of trypsin-split normal adult skin.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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LHP1
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Specificity
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This antibody is specific to human cytokeratin 10. It recognizes a 56.5 kD cytokeratin protein and found in suprabasal layers of keratinizing stratified epithelia, suprabasal layers of non- keratinizing stratified epithelia and in differentiated areas of squamous carcinomas. This antibody is useful in the differentiation of stratified squamous epithelia.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).
Leigh, Purkis, Whitehead et al.: "Monospecific monoclonal antibodies to keratin 1 carboxy terminal (synthetic peptide) and to keratin 10 as markers of epidermal differentiation." in: The British journal of dermatology, Vol. 129, Issue 2, pp. 110-9, 1995 (PubMed).
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Alternatives for antigen "Cytokeratin 10 (KRT10)", type "Antibodies"
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Hosts
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Mouse (23), Rabbit (10), Guinea Pig (1)
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Reactivities
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Human (30), Dog (Canine) (4), Rat (Rattus) (4), Cat (Feline) (2), Mouse (Murine) (2), Cow (Bovine) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Western Blotting (WB) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Immunofluorescence (IF) (9), Enzyme Immunoassay (EIA) (4), Flow Cytometry (FACS) (4), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (3), ELISA (2), Immunocytochemistry (ICC) (1)
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Epitopes
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N-Term (2), Center (1)
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