»
Antibodies
»
anti-Cytokeratin 10 (KRT10) antibody
Cytokeratin 10 (KRT10) antibody
| Antigen |
|
| Synonyms |
K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik |
| Clonality |
Monoclonal (MNF116) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
| Catalog no. |
ABIN98151 |
| Quantity |
6ml (1 to 4 ug/ml) (Variants) |
| Price |
135.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
Cytokeratin 10
|
|
Immunogen
|
BALB/C mice were immunized with crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells.
|
|
Format
|
Supernatant (prediluted)
|
|
Isotype
|
IgG1 kappa (Matching secondary antibodies)
|
|
Clone
|
MNF116
|
|
Specificity
|
This antibody recognizes keratin polypeptide of 45, 46 and 56.5 kD. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts and tracheal epithelium.
|
|
Application Notes
|
Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic
|
|
Concentration
|
1 to 4 ug/ml
|
|
Buffer
|
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
Extracellular Matrix
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
|
Publications
|
Norton, Thomas, Isaacson: "Cytokeratin-specific monoclonal antibodies are reactive with tumours of smooth muscle derivation. An immunocytochemical and biochemical study using antibodies to intermediate filament cytoskeletal proteins." in: Histopathology, Vol. 11, Issue 5, pp. 487-99, 1987 (PubMed).
Gatter, Abdulaziz, Beverley et al.: "Use of monoclonal antibodies for the histopathological diagnosis of human malignancy." in: Journal of clinical pathology, Vol. 35, Issue 11, pp. 1253-67, 1983 (PubMed).
|
Alternatives for antigen "Cytokeratin 10 (KRT10)", type "Antibodies"
|
Hosts
|
Mouse (23), Rabbit (10), Guinea Pig (1)
|
|
Reactivities
|
Human (30), Dog (Canine) (4), Rat (Rattus) (4), Cat (Feline) (2), Mouse (Murine) (2), Cow (Bovine) (1)
|
|
Applications
|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Western Blotting (WB) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Immunofluorescence (IF) (9), Enzyme Immunoassay (EIA) (4), Flow Cytometry (FACS) (4), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (3), ELISA (2), Immunocytochemistry (ICC) (1)
|
|
Epitopes
|
N-Term (2), Center (1)
|
