Keratin 17 (KRT17) antibody

Antigen: Keratin 17 (KRT17)

Synonyms: PC, K17, PC2, PCHC1, Krt1-17, MGC159887, Ka17, KRT17, krt16

Clonality: Monoclonal (E3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98159

Additional Information

Alternative name: Cytokeratin 17

Immunogen: Cytoskeletal fraction of rat colon epithelium.

Format: Supernatant

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: E3

Specificity: This antibody is specific to 46 kD cytokeratin protein designated cytokeratin 17. It reacts with basal cells in complex epithelia and reacts strongly with squamous cell carcinomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human squamous cell carcinoma stained with Cytokeratin 17 antibody

Publications

Publications: Smedts, Ramaekers, Troyanovsky et al.: "Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 140, Issue 3, pp. 601-12, 1992 (PubMed).

Wetzels, Schaafsma, Leigh et al.: "Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18." in: Histopathology, Vol. 20, Issue 4, pp. 295-303, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Troyanovsky, Guelstein, Tchipysheva et al.: "Patterns of expression of keratin 17 in human epithelia: dependency on cell position." in: Journal of cell science, Vol. 93 ( Pt 3), pp. 419-26, 1990 (PubMed).