Keratin 20 (KRT20) antibody

Antigen: Keratin 20 (KRT20)

Synonyms: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Clonality: Monoclonal (Q2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98182

Additional Information

Alternative name: Cytokeratin 20

Immunogen: BALB/C mice were immunized with partially purified human keratin 20 expressed in bacterial cells.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: Q2

Specificity: This antibody with a protein of 46 kD. Cytokeratin 20 is less acidic than other type I cytokeratins and is of interest due to its more restricted expression in tissue. Cytokeratin 20 is expressed primarily in gastric and intestinal epithelium, urothelium and Merkel- cells. Cytokeratin 20 is expressed in adenocarcinomas of colon, stomach, pancreas and the bile system.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Moll, Loewe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).