Cytokeratin 1 (KRT1) antibody

Antigen: Cytokeratin 1 (KRT1)

Synonyms: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Clonality: Monoclonal (KL1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98191

Additional Information

Alternative name: Cytokeratin

Immunogen: Human keratin isolated from epidermal stratum corneum.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: KL1

Specificity: This antibody is specific to 56 kD cytokeratin. Immunoblotting analysis revealed that this antibody reacts with cytokeratin 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18. This antibody stains with neoplastic cells of epithelial origin. Non-epithelial cells and skin basal layers cells do not stain with this antibody.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment prior to immunostaining of formalin fixed paraffin embedded tissue sections may enhance staining intensity. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin Pan antibody

Publications

Publications: Takahashi, Fujita, Okabe et al.: "Immunohistochemical characterization of basal cell adenomas of the salivary gland." in: Pathology, research and practice, Vol. 187, Issue 2-3, pp. 145-56, 1991 (PubMed).

Viac, Reano, Brochier et al.: "Reactivity pattern of a monoclonal antikeratin antibody (KL1)." in: The Journal of investigative dermatology, Vol. 81, Issue 4, pp. 351-4, 1983 (PubMed).

Wells, Heryet, Brochier et al.: "The immunocytochemical detection of axillary micrometastases in breast cancer." in: British journal of cancer, Vol. 50, Issue 2, pp. 193-7, 1984 (PubMed).