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Cytokeratin 1 (KRT1) antibody
| Antigen | Cytokeratin 1 (KRT1) |
| Synonyms | K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK |
| Clonality | Monoclonal (Lu-5) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Cow (Bovine), Rat (Rattus), Chicken, Snake, Frog |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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1 reference available |
| Catalog no. | ABIN98198 |
| Quantity | 1ml (100ug/ml approximately) (Variants) |
| Price | 282.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Cytokeratin |
| Immunogen | BALB/C mice were injected with Lung cancer cell line. |
| Clone | Lu-5 |
| Specificity | This antibody reacts with cytokeratins of acidic as well as basic subfamilies, i.e. 1,2,3,4,5,6,7,8,10,12,13,14,15,16,17,18 and 19. It reacts with keratinized and corneal epidermis, stratified epithelia, hyperproliferative keratinocytes and simple epithelia. This antibody is a broad spectrum anti pan cytokeratin antibody, which differentiates epithelial from non-epithelial tumors. It cross reacts with cow, rat , chicken, snake and frog. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require predigestion with Pepsin. Positive Control: Skin. Cellular localization: Cytoplasmic |
| Concentration | 100ug/ml approximately |
| Purification | Ig Fraction |
| Buffer | This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Images
| Formalin fixed paraffin embedded human squamous cell carcinoma of lung stained with Cytokeratin Pan antibody |
Publications
| Publications |
von Overbeck, Staehli, Gudat et al.: "Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5)." in: Virchows Archiv. A, Pathological anatomy and histopathology, Vol. 407, Issue 1, pp. 1-12, 1985 (PubMed).
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Alternatives
Alternatives for antigen "Cytokeratin 1 (KRT1)", type "Antibodies"
| Hosts | Mouse (28), Guinea Pig (2), Rabbit (2) |
| Reactivities | Human (27), Cow (Bovine) (2), Rat (Rattus) (2), Chicken (1), Frog (1), Mouse (Murine) (1), Snake (1) |
| Applications | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Western Blotting (WB) (11), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (4), Immunofluorescence (IF) (2), ELISA (1), Immunocytochemistry (ICC) (1) |
