Keratin Cocktail antibody

Antigen: Keratin Cocktail

Clonality: Monoclonal (DBS-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98199

Additional Information

Alternative name: Cytokeratin Cocktail

Immunogen: BALB/c mice were immunized with human epidermal stratum corneum and with human epidermal keratins.

Format: Liquid (prediluted)

Isotype: IgG1, IgG2a  (Matching secondary antibodies)

Clone: DBS-1

Specificity: This antibody cocktail has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas in lymph nodes and bone marrow. It reacts with cytokeratin polypeptide 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18 in immunoblott analyses. This antibody cocktail recognizes most of the acidic (Type I) keratins as well as basic (Type II). It recognizes keratin polypeptides of 40, 48, 50, 52, 54, 56.5, 58, 59 and 64 to 67 kD. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. This antibody can be used to stain distinct cell layers in an epithelial tissue and various neoplasms of epithelial origin.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. .

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Angus, Kiberu, Purvis et al.: "Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1." in: The Journal of pathology, Vol. 155, Issue 1, pp. 71-5, 1988 (PubMed).

Gatter, Abdulaziz, Beverley et al.: "Use of monoclonal antibodies for the histopathological diagnosis of human malignancy." in: Journal of clinical pathology, Vol. 35, Issue 11, pp. 1253-67, 1983 (PubMed).