Cytomegalovirus, Coctail antibody

Antigen: Cytomegalovirus, Coctail

Clonality: Monoclonal (DDG9-CCH2)

Host: Mouse

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98212

Additional Information

Alternative name: Cytomegalovirus (CMV) Cocktail

Immunogen: BALB/c mice were immunized with cytomegalovirus infected cell lysate.

Format: Supernatant (prediluted)

Isotype: IgG1, IgG2a kappa  (Matching secondary antibodies)

Clone: DDG9-CCH2

Specificity: This cocktail antibody reacts with an immediate early and early CMV antigens. The antibody stain infected cells giving a nuclear staining pattern with an immediate early and an early antigen. In Western blot, DDG9 reacts with 76 kD protein, where as CCH2 reacts with a 43 kD protein.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Infected cervix. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Virology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Leland, Hansing, French: "Clinical experience with cytomegalovirus isolation using conventional cell cultures and early antigen detection in centrifugation-enhanced shell vial cultures." in: Journal of clinical microbiology, Vol. 27, Issue 6, pp. 1159-62, 1989 (PubMed).

Ashley, Peterson, Abbo et al.: "Comparison of monoclonal antibodies for rapid detection of cytomegalovirus in spin-amplified plate cultures." in: Journal of clinical microbiology, Vol. 27, Issue 12, pp. 2858-60, 1990 (PubMed).

Niedobitek, Finn, Herbst et al.: "Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparison of methods." in: Journal of clinical pathology, Vol. 41, Issue 9, pp. 1005-9, 1989 (PubMed).