Dinitrophenol (DNP) antibody

Antigen: Dinitrophenol (DNP)

Clonality: Monoclonal (3E10)

Host: Armenian Hamster

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN98436

Additional Information

Immunogen: This monoclonal antibody was produced after repeated immunizations of armenian hamsters with DNP conjugated KLH.

Format: Liquid (sterile filtered)

Isotype: IgG  (Matching secondary antibodies)

Clone: 3E10

Description: Anti-DNP (ARMENIAN HAMSTER) Monoclonal Antibody.

Specificity: The antibody reacts with DNP but not with carrier protein.

Application Details

Application Notes: This monoclonal antibody reacts specifically with DNP (2,4-dinitrophenol). The antibody reacts with free DNP, DNP conjugated to carriers such as thyroglobulin or BSA, and detects the presence of DNP in proteins and nucleic acids. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Recommended Dilutions: ELISA: 1:2,000 - 1:10,000. WB: 1:500 - 1:3,000. IHC: 1:250 - 1:1,500. OTHER: User Optimized.

Concentration: 0.91 mg/ml (by UV absorbance at 280 nm)

Purity: This product is an IgG fraction antibody purified from ascites or tissue culture supernate by affinity chromatography followed by extensive dialysis against the buffer stated above. Assay by SDS-PAGE resulted in a single protein band.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Storage: Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Toxins

Restrictions: For Research Use only