E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody

Antigen: E2F Transcription Factor 4, P107/p130-Binding (E2F4)

Synonyms: E2F-4, AI427446, 2010111M04Rik, e2f4, MGC80324, E2F4, MGC139939, e2f-4

Clonality: Monoclonal (4E2F04)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98639

Additional Information

Alternative name: E2F-4 Transcription Factor

Immunogen: BALB/C mice were immunized with a recombinant human E2F-4 protein.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 4E2F04

Specificity: This antibody reacts with a protein of 56 kD. E2F

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear, cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with E2F-4 Transcription Factor antibod