Elastase antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
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Application
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Alternatives ELISA, Western Blotting (WB), Immunoprecipitation (IP)
ELISA (13), Western Blotting (WB) (11), Enzyme Immunoassay (EIA) (8), Radioimmunoassay (RIA) (8), Immunocytochemistry (ICC) (2), Immunoelectrophoresis (IEP) (2), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Flow Cytometry (FACS) (1), Functional Studies (Func) (1), Immunofluorescence (IF) (1)
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| Catalog no. |
ABIN98664 |
| Quantity |
2ml (80.0 mg/ml (by Refractometry)) (Variants) |
| Price |
309.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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Elastase [Porcine Pancreas].
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Format
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Lyophilized
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Application Notes
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Dilution: This product has been assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified antiRabbit IgG [HampL] [Goat] code 6111302 and [ABTS [22'azinobis[3ethylbenthiazoline6sulfonic acid]] code ABTS100 as a substrate for 30 minutes at room temperature. A working dilution of 1/5000 to 1/20000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Notice: suitable for immunoblotting [western or dot blot] ELISA immunoprecipitation and most immunological methods requiring high titer and specificity. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity.
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Concentration
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80.0 mg/ml (by Refractometry)
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Purity
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
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Purification
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This product was prepared from monospecific antiserum by a delipidation anddefibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partiallypurified Elastase [Porcine Pancreas]. Cross reactivity against Elastase from other tissues and species may occurbut have not been specifically determined.
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Storage
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Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (orequivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stablefor several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of restoration.
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Research Area
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Signaling, Extracellular Matrix, Proteases
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Restrictions
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For Research Use only
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Alternatives for antigen "Elastase", type "Antibodies"
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Hosts
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Rabbit (20), Mouse (5), Sheep (5)
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Reactivities
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Human (10), Pig (Porcine) (8), Mouse (Murine) (1), Rat (Rattus) (1)
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Applications
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ELISA (13), Western Blotting (WB) (11), Enzyme Immunoassay (EIA) (8), Radioimmunoassay (RIA) (8), Immunocytochemistry (ICC) (2), Immunoelectrophoresis (IEP) (2), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Flow Cytometry (FACS) (1), Functional Studies (Func) (1), Immunofluorescence (IF) (1)
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Conjugates
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Biotin (5), HRP (1)
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