Epithelial Antigen antibody

Antigen: Epithelial Antigen

Clonality: Monoclonal (Ber-EP4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98879

Additional Information

Immunogen: BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.

Format: Supernatant

Isotype: IgG1 kappa

Clone: Ber-EP4

Specificity: Epithelial antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Kidney. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Momburg, Moldenhauer, Haemmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Beer, Shepherd, Theaker: "Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin." in: Histopathology, Vol. 37, Issue 3, pp. 218-23, 2000 (PubMed).

Carella, Deleonardi, DErrico et al.: "Immunohistochemical panels for differentiating epithelial malignant mesothelioma from lung adenocarcinoma: a study with logistic regression analysis." in: The American journal of surgical pathology, Vol. 25, Issue 1, pp. 43-50, 2000 (PubMed).

Hosch, Kraus, Scheunemann et al.: "Malignant potential and cytogenetic characteristics of occult disseminated tumor cells in esophageal cancer." in: Cancer research, Vol. 60, Issue 24, pp. 6836-40, 2001 (PubMed).