Mucin 1, Cell Surface Associated (MUC1) antibody

Antigen: Mucin 1, Cell Surface Associated (MUC1)

Synonyms: EMA, PEM, PUM, KL-6, MAM6, PEMT, CD227, H23AG, MGC188069, MUC1

Clonality: Monoclonal (GP1-4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98889

Additional Information

Alternative name: Epithelial Membrane Antigen (EMA)

Immunogen: BALB/C mice were immunized with human milk fat globule membranes.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: GP1-4

Specificity: This antibody reacts with an antigen of 265-400 kD belonging to a heterogeneous group of heavily glycosylated proteins called human milk fat globule proteins. It stains both normal and neoplastic cells. Among normal epithelia, it reacts strongly with mammary epithelium and glandular epithelia but shows a patchy staining with squamous epithelium.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is not necessary but in some cases it may enhance the immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cell membrane and cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with EMA antibody

Publications

Publications: Pinkus, Kurtin: "Epithelial membrane antigen--a diagnostic discriminant in surgical pathology: immunohistochemical profile in epithelial, mesenchymal, and hematopoietic neoplasms using paraffin sections and monoclonal antibodies." in: Human pathology, Vol. 16, Issue 9, pp. 929-40, 1985 (PubMed).

Cordell, Richardson, Pulford et al.: "Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry." in: British journal of cancer, Vol. 52, Issue 3, pp. 347-54, 1985 (PubMed).

Heyderman, Strudley, Powell et al.: "A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2." in: British journal of cancer, Vol. 52, Issue 3, pp. 355-61, 1985 (PubMed).