Epstein-Barr Virus (EBV) Cocktail antibody

Antigen: Epstein-Barr Virus (EBV) Cocktail

Clonality: Monoclonal (CS1 CS2 CS3 CS4)

Host: Mouse

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN98940

Additional Information

Immunogen: Recombinant fusion protein containing sequences of bacterial beta-galactosidase and EBV encoded latent membrane protein.

Format: Supernatant (prediluted)

Isotype: IgG1  (Matching secondary antibodies)

Clone: CS1 CS2 CS3 CS4

Specificity: This antibody is specific to 60 kD latent membrane protein (LMP-1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic c-terminus of the cytoplasmic domain of LMP-1. This antibody stains strongly with EBV positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Infected lymphoblastoma. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Virology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Jarrett, Gallagher, Jones et al.: "Detection of Epstein-Barr virus genomes in Hodgkin's disease: relation to age." in: Journal of clinical pathology, Vol. 44, Issue 10, pp. 844-8, 1992 (PubMed).

Pallesen, Hamilton-Dutoit, Rowe et al.: "Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkin's disease." in: Lancet, Vol. 337, Issue 8737, pp. 320-2, 1991 (PubMed).

Rowe, Evans, Young et al.: "Monoclonal antibodies to the latent membrane protein of Epstein-Barr virus reveal heterogeneity of the protein and inducible expression in virus-transformed cells." in: The Journal of general virology, Vol. 68 ( Pt 6), pp. 1575-86, 1987 (PubMed).