Excision Repair Cross Complementing Polypeptide-1 (ERCC1) antibody

Antigen: Excision Repair Cross Complementing Polypeptide-1 (ERCC1)

Clonality: Monoclonal (8F1)

Host: Mouse

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN99004

Additional Information

Immunogen: BALB/C mice were injected with full length recombinant human ERCC1 protein.

Format: Ig-fraction

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 8F1

Specificity: This antibody reacts with a 33- 36 KDa protein known as ERCC1 (Excession Repair Cross Complementing) polypeptide. ERCC1 is required for nucleotide excision repair of damaged DNA and is homologous to RAD10. In mammalian cells, XPG cleaves 3

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western Blotting (1:100-500).

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with ERCC1antibody

Publications

Publications: Miura, Nakamura, Sasaki et al.: "Roles of XPG and XPF/ERCC1 endonucleases in UV-induced immunostaining of PCNA in fibroblasts." in: Experimental cell research, Vol. 226, Issue 1, pp. 126-32, 1996 (PubMed).

Hayashi, Takao, Tanaka et al.: "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines." in: Mutation research, Vol. 407, Issue 3, pp. 269-76, 1998 (PubMed).