Ezrin (EZR) antibody
| Antigen |
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| Synonyms |
CVL, CVIL, VIL2, MGC1584, FLJ26216, DKFZp762H157, EZR, vil2, wu:fc05c03, wu:fj31d06, cvl, cvil, MGC97499 |
| Clonality |
Monoclonal (3C12) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (65), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (5), ELISA (Detection) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN99023 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
492.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Ezrin
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Immunogen
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BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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3C12
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Specificity
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This antibody with a protein of 70 kD. Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF- stimulated cells. Ezrin is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. This antibody cross reacts with monkey, cow, kangaroo, rat, mouse and hamster.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Lung. Cellular localization: Cell membrane Other Applications: Immunoprecipitation, Western blotting (1:100-750).
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Boehling, Turunen, Jaeaeskelaeinen et al.: "Ezrin expression in stromal cells of capillary hemangioblastoma. An immunohistochemical survey of brain tumors." in: The American journal of pathology, Vol. 148, Issue 2, pp. 367-73, 1996 (PubMed).
Helander, Carpuen, Turunen et al.: "ICAM-2 redistributed by ezrin as a target for killer cells." in: Nature, Vol. 382, Issue 6588, pp. 265-8, 1996 (PubMed).
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Alternatives for antigen "Ezrin (EZR)", type "Antibodies"
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Hosts
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Rabbit (71), Goat (7), Mouse (6)
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Reactivities
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Human (82), Mouse (Murine) (62), Rat (Rattus) (54), Dog (Canine) (5), Cow (Bovine) (3), Hamster (2), Chicken (1), Rabbit (1)
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Applications
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Western Blotting (WB) (65), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (5), ELISA (Detection) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr566 (6), pTyr353 (4), pThr567 (3), pSer566 (1), pTyr146 (1), pTyr478 (1)
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