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Antibodies
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anti-Coagulation Factor VIII (F8) (Factor 8) antibody
Coagulation Factor VIII (F8) (Factor 8) antibody
| Antigen |
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| Synonyms |
AHF, F8B, F8C, HEMA, FVIII, DXS1253E, F8, Cf8, Cf-8, fb61d02, wu:fb61d02 |
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Epitope
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| Clonality |
Monoclonal (F8-86) |
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Host
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Reactivity
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Application
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Western Blotting (WB) (42), Enzyme Immunoassay (EIA) (29), ELISA (22), To be determined by user (TBD) (7), ELISA (Detection) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (IHC) (3), Functional Studies (Func) (1), Immunodiffusion (ID) (1), Immunoelectrophoresis (IEP) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN99037 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
276.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Factor VIII (Factor 8)
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Immunogen
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Von Willebrand factor isolated from human plasma.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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F8-86
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Specificity
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This antibody reacts with Von Willebrand factor in endothelial cells. It also reacts with megakaryocytes in human bone marrow. Proteolytic enzyme digestion of formalin fixed paraffin embedded tissue sections is required prior to immunostaining.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Positive Control: Tonsil. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Coagulation
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Hruban, Kuhajda, Mann: "Acute myelofibrosis. Immunohistochemical study of four cases and comparison with acute megakaryocytic leukemia." in: American journal of clinical pathology, Vol. 88, Issue 5, pp. 578-88, 1987 (PubMed).
Naiem, Gerdes, Abdulaziz et al.: "The value of immunohistological screening in the production of monoclonal antibodies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 145-60, 1982 (PubMed).
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Alternatives for antigen "Coagulation Factor VIII (F8)", type "Antibodies"
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Hosts
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Mouse (44), Sheep (11), Rabbit (9), Rat (5), Chicken (1)
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Reactivities
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Human (50), Mouse (Murine) (7), Dog (Canine) (2), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (42), Enzyme Immunoassay (EIA) (29), ELISA (22), To be determined by user (TBD) (7), ELISA (Detection) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (IHC) (3), Functional Studies (Func) (1), Immunodiffusion (ID) (1), Immunoelectrophoresis (IEP) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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HRP (2), Alkaline Phosphatase (AP) (1), Biotin (1), FITC (1)
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Epitopes
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Factor 8c (24), A2 domain (3), Factor 8 (1), Heavy chain (1)
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Alternatives 
